|
|
Interaction of polyomaviruses with proteasomal system of host cell
Verdánová, Martina ; Drda Morávková, Alena (advisor) ; Horák, Martin (referee)
Interaction of polyomaviruses with proteasomal system of host cells Abstract: Viral family Polyomaviridae includes besides model organisms - mouse polyomavirus and SV40 virus, also human pathogens, for example, BK virus. Polyomaviruses are small non- enveloped viruses with double-stranded DNA. Understanding of their life cycle is important for their use in gene therapy and immunotherapy as well as for prevention and treatment of complications caused by these viruses. This thesis is focused on early phases of MPyV and SV40 infection studying, mainly on delivery of viral genome to nucleus and role of proteasomal system in this stage of infection. It was found out that inhibition of proteasomes by specific inhibitor leads to increase of early non-structural protein LT expression, which was chosen as marker for viral entry to the nucleus and successful viral expression. Relative localization of proteasomes and VP1 protein of MPyV and SV40 was monitored and it showed 10% colocalization of mentioned structures. Further, it was found out that proteasomal inhibitor MG-132 negatively influences the replication of both viral and cellular DNA. Next aim of this diploma thesis was to prepare antigen - unique part of VP2 protein of BKV - for producing antibody. Expression vector with inserted fragment of unique part of...
|
|
|
Properties and functions of agnoprotein of polyomaviruses
Zosinčuková, Tereza ; Forstová, Jitka (advisor) ; Vinšová, Barbora (referee)
Polyomaviridae family includes small DNA viruses with simple structure and a small genome encoding only a few proteins. These proteins include large T and small T antigens, as well as 2 to 3 structural proteins known as VP1, VP2 and VP3. In addition, some members of the Polyomaviridae family encode in their genome a small non-structural protein called agnoprotein. Among human polyomaviruses, agnoprotein is present in BK polyomavirus and JC polyomavirus. These viruses are the causative agents of some serious diseases in immunocompromised humans and therefore, they are the subject of intensive research. Simian vacuolating virus 40 is another example of a virus which encodes the agnoprotein. Agnoprotein is capable to manipulate its host cell, disrupt vesicle transport and is also crucial for viral replication and transcription. It appears to play an important role in the morphogenesis of virions and/or in their release from the cell. This paper comprehensively summarizes the latest insights into the properties and functions of the agnoprotein BK polyomavirus, JC polyomavirus and SV40 virus, focusing on the production of this protein during infection, its structure, posttranslational modifications, cell localization, interaction partners and the overall importance of this enigmatic protein for the...
|
|
|
Interaction of polyomaviruses with proteasomal system of host cell
Verdánová, Martina ; Drda Morávková, Alena (advisor) ; Horák, Martin (referee)
Interaction of polyomaviruses with proteasomal system of host cells Abstract: Viral family Polyomaviridae includes besides model organisms - mouse polyomavirus and SV40 virus, also human pathogens, for example, BK virus. Polyomaviruses are small non- enveloped viruses with double-stranded DNA. Understanding of their life cycle is important for their use in gene therapy and immunotherapy as well as for prevention and treatment of complications caused by these viruses. This thesis is focused on early phases of MPyV and SV40 infection studying, mainly on delivery of viral genome to nucleus and role of proteasomal system in this stage of infection. It was found out that inhibition of proteasomes by specific inhibitor leads to increase of early non-structural protein LT expression, which was chosen as marker for viral entry to the nucleus and successful viral expression. Relative localization of proteasomes and VP1 protein of MPyV and SV40 was monitored and it showed 10% colocalization of mentioned structures. Further, it was found out that proteasomal inhibitor MG-132 negatively influences the replication of both viral and cellular DNA. Next aim of this diploma thesis was to prepare antigen - unique part of VP2 protein of BKV - for producing antibody. Expression vector with inserted fragment of unique part of...
|
guest ::
